Accurate microfluidic dosing is essential for life science and genomics research, as well as the administration of medical care. For OEMs developing microfluidic dosing machines, accurate valve control ensures precise results. However, to specify the optimum valve system, consideration over criteria from material compatibility to manifold integration is vital to ensure repeatable accuracy.
Troy Stehr, Industry Account Manager for Lab & MedTech applications at fluid control specialist Bürkert, discusses requirements for accurate microfluidic control.
Reagents are integral to research in life sciences, and the result of how they chemically react with other substances, by measuring, detecting, or examining an outcome, is vital for the development of medical treatments. Essential to this process is accurate, repeatable control of reagent dosing, in microfluidic volumes down to 1µl and below. These same requirements apply to genomics, such as conducting a polymerase chain reaction (PCR) technique to amplify select DNA segments. Accurate and reliable microfluidic dosing can also be life dependent for many medical applications.
Today, machines can replace manual handling of pipettes with more accurate and consistent dosing. Automation not only saves the time of skilled humans, but fast dosing of multiple channels improves productivity too. To achieve these results, there’s high reliance on a dosing machine’s valve control system.
Open or closed loop?
Understanding the required dosing accuracy is typically the starting point in valve system specification. Patients are treated or diagnosed based on lab results, so the accuracy requirement of the intended assessment determines the dosing precision the machine has to provide. Optimising volume use is also important to control costs, both in the use and the disposal of some very expensive reagents and involved substances.
Sufficient for many microfluidic applications, time-pressure dosing provides precision of <1% with repeatable volumetric accuracy between 2-5%. With this technique, media flow is controlled by valve opening or closing for a defined time for each switching cycle. Using air or an inert gas to pressurise the fluid reservoir, combined with a dosing valve, this technique enables controlled dosing down to single digit µl (microlitre) volumes.
Bürkert’s microvalves for time pressure dosing are capable of ~10μs (microsecond) control signal steps for high accuracy valve control lower than 1%. If even greater accuracy is required, Bürkert also provides closed loop valve control. These systems incorporate a sensor, providing feedback on actual valve status that enables constant adjustment to valve opening/closing rates. Alternatively, an open loop system, like time-pressure dosing, operates purely on the valve’s input command, irrespective of changes that could impact its switching time, and as a result, the actual dosing volume.
μl and nl dosing accuracy
Closed loop systems often operate on the Coriolis principle, where vibrations of a liquid-carrying tube are measured with a resulting flow-proportional output. With this technology, Bürkert’s mass flow controller/meter can achieve dosing accuracy at levels below 1ml. However, a new innovation is set to become the first device available on the market with μl (microlitre) measurement and closed loop control accuracy, with a differential pressure sensor integrated in a device together with a valve. Bürkert’s new dosing system measures and controls drops down to 50nl (nanolitres), with closer than 1% accuracy measured over millions of cycles completed in trials so far.
Crucial to control over time is compatibility between the media and the material fluid block and seals, especially when multiple reagents and substances are involved. Depending on the media properties, different seal materials might be required and their selection requires careful consideration. Compatibility challenges can result in swollen seals, through to rupture over time, and when dealing with microfluidics, resulting control problems are amplified. Within Bürkert’s system, PEEK, used for valve bodies, and the sealing material FFKM or EPDM are the only wetted materials.
Precision-engineered manifolds
To separate and control media flow, the valve system requires a manifold array with the number of inlets and outlets to suit the variety of required substances. Incorporating bespoke manifold design and manufacture with the selected valve is advantageous, particularly for microfluidic applications, to ensure optimised pressure management and heat exchange properties. As per valve design, the manifold is also subject to materials compatibility, so relying on the same vendor gives greater security in an efficient outcome. Bürkert precision engineers bespoke, plastic injection moulded manifolds specific for microfluidic control applications.
To optimise productivity, 10 or even more channels might be required, with high-speed dispensing. Combined with repeatable accuracy, to achieve system reliability over millions of valve cycles, a remote diagnostics capability, enabled by Industry 4.0 Ethernet communications, confirms actual valve operation data. Not only does this capability remove the time required for a physical human check, it provides a certainty, unattainable by a manual inspection alone, that the well plates are filled with the right volume and proportion of reagents.
Bürkert Fluid Control Systems : Kirsty Anderson
Tel: +44 (0)1285 648761 Fax: +44 (0)1285 648721
Email: kirsty.anderson@burkert.com
Web: www.burkert.co.uk
Burkert Fluid Control Systems
- 01285648720
- sales.uk@burkert.com
- http://www.burkert.com/
- Fluid Control Centre 1 Bridge End Gloucestershire Cirencester GL7 1QY GB
About us
Bürkert is present in thirtyfive countries around the world. We also work with a large network of distributors and partners, which means we are as close as possible to our customers. This global presence ensures full service and support to all of our customers in every country around the world. Research is the lifeblood of our company.
At Bürkert, we are never satisfied with the status quo and are continually seeking new technologies and solutions for our customers. Every year, our people develop new and highly advanced products and solutions, ranging from integrated process measurement and control units to the most sophisticated systems used in pharmaceutical research. To be a market leader, we are also an R&D leader.
Therefore, our investment in research & development is one of the highest in our industry. In our research centres in Germany and France, 150 people are committed to working for a common future for our company and our customers. We are committed to offering our expertise wherever it is needed, anywhere in the world. This global presence ensures that our advances in fluid control technology are also global.
What we do in a nutshell
Manufacture of process equipment. One of the few manufacturers to provide solutions for the complete control loop.
Where we supply to
UK Ireland, Europe
Industries we supply to
Food and Beverage, Pharmaceutical Cosmetics Toiletries, Water and Wastewater
-
Surface Acoustic Wave (SAW) Technology: Advanced Flow Measurement and Biofilm Prevention in WFI Loops
Bürkert, a leader in flow control solutions, has introduced an innovative flowmeter that employs Surface Acoustic Wave (SAW) technology. This...
-
Bürkert Launches Ultra High-pressure Valves For Hydrogen Applications
Liquid and gas flow control specialist Bürkert has launched a range of valves designed to control ultra high-pressure flow for...
-
A Question Of Reliability: Supply And Control Devices For Industrial Printers
In the world of industrial and commercial printing, the efficiency and precision of ink supply and control systems are crucial...
-
UK’s first hydrogen kiln test rig helps manufacturers make a sustainable switch
A hydrogen-fuelled kiln test rig is helping brick and ceramics manufacturers to support the energy transition. In a UK first,...
-
Bürkert’s OALAB simplifies chlorine measurement for industrial carbon filters
Common in pharmaceutical, food and beverage applications, industrial high-rate granular activated carbon (GAC) filters remove chlorine from water supplies, safeguarding...
-
Tradition benefitting from technology: Controlling the brewing process
The food and beverage industry is continually evolving, driven by the need for enhanced efficiency, quality, and safety. Central to...
-
FLOWave | Oil dosing (English)
-
Feldbus Gateway Modul ME43: How to operate FLOWave (DE)
